The Replication Of Dna By Escherichia Coli Essay - 1479 Words

Introduction Today’s issue of Biology: Meselson and Stahl (Vol 4, 1958) includes a groundbreaking development into further understanding of DNA Replication. On pages 671 – 682 is an article titled â€Å"The Replication of DNA in Escherichia Coli†. Meselson and Stahl conducted an experiment to understand how DNA self-replicates by the use of Bacterial transformation to clone parental DNA. In the article, Meselson and Stahl investigate the distribution between the parental and daughter DNA molecules. This is achieved by the use of Radio Isotopic labelling. Uniform Isotopic N15 was grown in E.coli consisting of N14 medium to observe the distribution of N15 DNA macromolecules. Within the article, the authors also discuss the problems they faced during the investigation and how significant Watsons and Cricks Double helix suggests how DNA self-replicates. What is DNA? The discovery of the structure of DNA has always been associated with Watson and Crick. Their double helix structure, which they developed in 1983, has been used as a model to understand how DNA self-replicates (Karp, 2009). The structure of DNA is made up of covalent bonds between 4 unique nucleotide bases. These bases bind together via complementary base pairing, therefore allowing Adenine to only bind with Thymine, and Cytosine with Guanine (Penn State, Ebery College of Science, 2016). The use of covalent bonds makes the structure strongly bound like a zipper. However, just like a zipper, it can easily open upShow MoreRelatedHow Do Bacteria Divide?1600 Words   |  7 Pagesto explain some aspects of DNA replication and septation – including the synthesis of peptidoglycan in the new cell wall – using the model organism Escherichia coli. Also, I will discuss the role and function of Fts proteins in cell division. Finally, I will discuss some less common forms of bac terial cell division, cell division where FtsZ is absent and some key facts from reliable sources on all the topics covered in this essay. Binary fission Before DNA replication E.coli cells prepare for cellRead MoreEscherichia Coli : Is A Well Studied Gram Negative Bacteria916 Words   |  4 PagesEscherichia coli K12 is a well-studied gram-negative bacteria. First isolated from the human gut, is one of the most used in molecular studies, being the knowledge obtained from studies of E. coli possible to apply to other organisms (Burton and Kaguni, 1997). Some enzymes have a crucial role in the transcription control, the topoisomerases enzymes play an important role in the level of DNA supercoiling, an important property of DNA and chromatin (Gilbert and Allan, 2014). The supercoiling levelRead MoreGenetic Engineering : Escherichia Coli1789 Words   |  8 Pagesorganism by manipulating its deoxyribonucleic acid (DNA). Bacteria possess plasmids which can be used as vectors for bacterial transformation. The plasmids can be altered to include donor DNA thus altering the physiological or physical characteristics of the bacterium. Escher ichia coli have been found to be the most effective bacterium in genetic transformation and heterologous expression of human genes or proteins. It was hypothesised that Escherichia coli HB101 K12 could be transformed with a pGLO plasmidRead MoreLara Guvelioglu. Bi 108 E2. Nahomie Rodriguez-Sastre. 04/13/17.1632 Words   |  7 Pages04/13/17 Bacteria Escherichia coli’s Genetic Transformation Using Vector Plasmid DNA pGLO Abstract The field of biotechnology involves the concept of genetic engineering, altering the DNA/genetic material of an organism using information from a different one. The process in which bacteria can obtain this manipulated genetic information from another source is called genetic transformation. The goal of this experiment was to genetically transform Escherichia coli bacteria’s DNA by inserting the vectorRead MoreMeselson and Stahl1272 Words   |  6 PagesFranklin Stahl are two biologists who prove that DNA replication was semiconservative. At the time, many strong evidences from experiments using bacterial viruses had already convinced most scientists that DNA was the molecule of heredity; however they knew little about the DNA replication process. After the dimensionally accurate model building by Watson and Crick, it was clear that the process of replication and information distribution have to use the DNA from parent cell as template to achieve anRead MoreEscherichia Coli Transformation For Ampicillin Resistance And Gfp Expression Using Pglo Plasmid And Calcium Chloride Transformation Solution1768 Words   |  8 Pages4/6/16 BI108 Lab D8 Escherichia coli transformation for ampicillin resistance and GFP expression using pGLO plasmid and calcium chloride transformation solution Abstract Within the growing field of biotechnology, genetic engineering is becoming more important than ever. To illustrate an application of genetic transformation, pGLO plasmid containing the reporter gene GFP, an arabinose operon, and a gene coding for ampicillin resistance was used to genetically transform Escherichia coli. Plates of LB/ampRead MoreDiscussion Questions On The Difference Between Genomic And Plasmid Dna796 Words   |  4 Pagesinfections, however numerous strains have been traced to the blood, urine and feces. Question #2 Plasmid DNA is an autonomously replicating circular DNA molecule that is only about 1,000-20,000 base pairs in size and is separate from bacterial chromosomes. Plasmids are a significant part of molecular biology because they are small enough to be cloned or modified, they endure self-replication, they are stable over long periods of time and they have a diverse range of gene expression. Having theseRead MoreEscherichia Coli ( Coli ) For Antimicrobial Resistance Genes1523 Words   |  7 PagesAnalysis Of Escherichia Coli (E. coli) For Antimicrobial Resistance Genes Hetvi .B. Patel Harper College ABSTRACT This essay reviews a basic microbiological research project that focused on identifying pathogenic strains of Escherichia coli (E. coli) from the poultry industry in a developing country such as Romania. The research methodology, results and its practical applications are discussed. 51 isolates of E. coli were taken from various samples of raw poultry meat and testedRead MoreFunction: of DNA polymerase500 Words   |  2 PagesFunction: of DNA polymerase DNA polymerase is a very important enzyme when it comes to DNA replication. DNA polymerase, more specifically, is involved in the process of reading and adding nucleotides to the DNA strand so a complimentary stand can be made. During the DNA replication process DNA polymerase puts new nucleotides on the 3’ end of the DNA Strand. Not only does DNA polymerase add nucleotides to a DNA strand it can also act somewhat as a â€Å"proof reader†. It can pause the replication processRead MoreHeritable Genetic Information In Escherichia Coli In Transformation1664 Words   |  7 PagesGenetic Information in Escherichia Coli in Transformation and Deoxyribonucleic Acid A report for Biology 2000 written by Alana Pay : 001196733 Introduction Griffin was a scientist that showed that there was a non-living component to heritability between organisms, although he had not yet discovered what exactly was the heritable component. However, during this time period, it was a common belief that the heritable material was contained within the protein of the organism, not the DNA that is now held